Does the Limit of detection play a role in deciding whether the test is positive, or negative Let us say that we have Joe who has recently been exposed to Covid-19.
Joe got himself tested by a normal PCR test on the second day of exposure and got a negative result. So, Joe does not worry about his condition, and he is going on a regular routine. He is going out and working with his colleagues. After 4 days Joe decided to get himself tested with CoviLamp-PCR, the results came positive while the normal PCR test results indicated Joe was negative for Covid-19 infection. negative with the.
To clarify the false positive and false negative results, he was again tested with a normal PCR test on the next day. He became more positive and realized he was spreading the virus to the people he was engaged with.

Scientific explanation

Discrepancy in the test results can be explained using following criteria’s

Variation In Sample

Normal PCR test usually uses oropharyngeal swabs as compared to CoviLamp’s saliva or nasal. Scientific literature indicates that patients from whom saliva was collected tested positive at a significantly higher rate than those where the sample came from NS.

Selectivity

CoviLamp enhances genetic material amplification efficiency with six primers for target genome amplification, surpassing standard PCR tests that rely on two primers and a probe, marking a significant advancement in diagnostic capabilities.

Variation In Sensitivity

CoviLamp limit of detection is between 6.25copies/microliter to 12.5 copies as compared to normal PCR test which has a LOD of approximately 30-40 copies/microliter Our LOD studies have been validated by 3 rd party CLIA certified; FDA recognized lab

Variation In Extraction /Elution Volume

CardiAI concentrates samples in a 25-microliter elution volume, yielding a higher template (CardiAI target gene) concentration. This accelerates threshold attainment, allowing for quicker negative classification in various PCR tests compared to diluted templates.